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A Word About Tests

Test kits are purchased at our office. You will be instructed how to collect and package the samples. The test kits are returned to our office. We then contact either FedEx or UPS to ship the packages to the labs. If you are an out of town patient, tests can be shipped to and from your home.  The labs take about a month to process the samples and send us the results. I will then meet with you to discuss the results and your treatment options.

Thank you for considering these valuable testing methods. My goal is to find and treat the cause of your symptoms, not to treat the symptoms themselves.

-Greg Brennan, D.C.

Adrenal Stress Profile

This test is used to evaluate the adrenal glands and hormone balance. In the event of extended/severe emotional, dietary, or pain/inflammation stressors, the brain-adrenal communication pathways are damaged and the adrenal glands do not function properly. Pregnenolone, the precursor to all other steroidal hormones, is diverted to produce cortisol. This is to the detriment of all other steroidal hormones (such as progesterone, DHEA and its metabolites, including testosterone and the estrogens). As pregnenolone is diverted to cortisol, DHEA depletion begins. Measuring cortisol levels and DHEA levels help to determine the severity and duration of chronic stressors. Treatment includes lifestyle modification and supplementation.

This is a saliva-based test. You will be asked to collect 1 teaspoon of saliva into four, provided test tubes throughout one day: one from 6-8 AM, one from Noon-1 PM, one from 4-5 PM, and one from 10-12 PM.


G. I. Pathogen Test 

This test is used to evaluate the presence of G.I. pathogens such as H. pylori, Campylobacter, C. difficile. This test can also detect the overgrowth of normal bacteria indicating an unhealthy G.I. environment. Treatment includes dietary changes and herbal, cleansing supplementation.

This is a stool-based test. You will be asked to collect a few milliliters of stool into 4-5 small provided, plastic containers on three days.


Organic Acids Test

This test measures the quantities of organic acids. Organic acids are the metabolites of the body’s chemical processes. This test is analogous to testing the exhaust of a car, which helps you understand what’s happening inside the engine. This test also provides an accurate evaluation of intestinal yeast and bacteria. Abnormal levels of these microorganisms can cause or worsen behavioral disorders, fatigue, and immune functions. Many people with chronic illnesses often excrete several abnormal organic acids. If abnormalities are detected, treatment including lifestyle modification and supplements such as vitamins and minerals are indicated.

This is a urine-based test. You will be asked to collect your morning urine to be placed into a provided, small plastic vial.


Dutch Test

The DUTCH test is a urine steroid hormone profile that measures hormones and hormone metabolites (called conjugates) in a dried urine sample, and is performed from the comfort of your home. It is the most cutting-edge way to truly see what’s going on when it comes to your hormones, because it doesn’t just measure hormones, but also something called “metabolites”, which are a measurement of hormone production and hormone breakdown.

Measuring both hormones and their metabolites can give you or your health care practitioner a much better overall picture of hormone production. For example, a DUTCH urine steroid hormone profile on someone with low salivary cortisol could show normal cortisol production, but high levels of metabolites. In other words, this would indicate that you are producing enough cortisol, but it’s just getting broken down into its metabolites very quickly. There are also some metabolites that are important markers for cancer risk that can only be measured in urine.

With serum (blood) and saliva hormone spot-testing, it’s possible to track variations in hormone release throughout the day – and this is a great way to measure how your hormones change during a 24-hour period (your circadian rhythm). In contrast, a standard 24-hour urine collection many physicians use reflects your total hormone output in a 24-hour period.

But by using the DUTCH urine steroid test, you get the best of all worlds: blood, saliva and urinary results with just a urine collection.

The DUTCH test measures the following:

  • Free cortisol
  • Free cortisone
  • Creatinine
  • Tetrahyrdocortisone
  • a- tetrahydrocortisol
  • b- tetrahydrocortisol
  • DHEAs
  • Progesterone metabolites (a-pregnanediol, b-pregnanediol)
  • Androgen metabolites (DHEAS, etiocholanolone, androsterone, testosterone, 5a-DHT, 5a-androstanediol, 5b-androstanediol, epi-testosterone)
  • Estrogen metabolites (estrone, estradiol, estriol, 2-OH- estrone, 4-OH- estrone, 16-OH-estrone, 2-Methoxy- estrone, 2-OH- estradiol)
  • 6- OH-melatonin- sulfate

For further information download the Steroid Pathway Diagram.